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Displaying record number 1354

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MAb ID	J1
HXB2 Location	Tat	Tat Epitope Map
Author Location	Tat(1-15)
Epitope
Subtype	B
Ab Type	N-term
Neutralizing	P
Species (Isotype)	human(IgG1λ)
Patient
Immunogen	vaccine
Keywords	antibody binding site, antibody sequence, subtype comparisons

Vaccine Details

Vaccine strain	B clade
Vaccine component	Tat

Notes

Showing 2 of 2 notes.

J1 database note: There are two distinct antibodies named J1 in the database.
J1: J1 is a single-chain fragment-variable scFv antibody derived from a Tat-toxoid vaccinated uninfected volunteer. J1 binds strongly to soluble rTAT protein and to denatured rTAT, suggesting that the epitope is linear. J1 recognized HIV-1 clade B Tat proteins Bru and HXB2, but did not bind to clade E (CM240), clade C (92Br), clade D (Eli) and clade A (Ug11RP). J1 inhibited Tat-transactivation of viral replication. Of three scFv antibodies, all bound the N-terminal amino acids 1-15, but G1 and G2 had kappa light chains and J1 had lambda, and the CDR3 of each was distinct, with J1's CDR3 sequence being: RDRYCSSPGCYKGADGGRLKDY. Moreau2004 (antibody binding site, subtype comparisons, antibody sequence)

References

Showing 1 of 1 references.

Moreau2004 Emmanuel Moreau, Johan Hoebeke, Daniel Zagury, Sylviane Muller, and Claude Desgranges. Generation and Characterization of Neutralizing Human Monoclonal Antibodies against Human Immunodeficiency Virus Type 1 Tat Antigen. J. Virol., 78(7):3792-3796, Apr 2004. PubMed ID: 15016898. Show all entries for this paper.