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Displaying record number 1336

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MAb ID	polyclonal
HXB2 Location	Env	Env Epitope Map
Author Location	Env
Epitope
Subtype	multiple
Ab Type
Neutralizing
Species (Isotype)	rabbit
Patient
Immunogen	vaccine
Keywords	subtype comparisons, vaccine antigen design

Vaccine Details

Vaccine type	protein
Vaccine strain	B clade IIIB, A clade UG37, B clade HAN2, D clade UG21, F clade BR29
Vaccine component	gp140, gp120ΔV1, V2, and V3
Adjuvant	Complete Freund's Adjuvant (CFA), Incomplete Freund's Adjuvant (IFA)

Notes

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A set of oligomeric envelope proteins were made from six primary isolates for potential use as vaccine antigens: 92/UG/037 (clade A), HAN2/2 (clade B), 92/BR25/025 (clade C), 92/UG/021 (clade D), 93/BR/029 (clade F) and MVP5180 (clade O). This was one of a panel of MAbs used to explore folding and exposure of well characterized epitopes. The clade C isolate BR25 is apparently misfolded, as conformation-dependent antibodies did not bind to it. Polyclonal sera raised in rabbits against the A, B, D and F antigens, which were deemed pure enough for immunization, as well as IIIB and IIIB with the V1, V2 and V3 loops deleted, cross-bound the other antigens, so shared epitopes across clades, but none of the sera had neutralizing activity. Jeffs2004 (vaccine antigen design, subtype comparisons)

References

Showing 1 of 1 reference.

Jeffs2004 S. A. Jeffs, S. Goriup, B. Kebble, D. Crane, B. Bolgiano, Q. Sattentau, S. Jones, and H. Holmes. Expression and Characterisation of Recombinant Oligomeric Envelope Glycoproteins Derived from Primary Isolates of HIV-1. Vaccine, 22(8):1032-1046, 25 Feb 2004. PubMed ID: 15161081. Show all entries for this paper.