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Displaying record number 1223

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MAb ID	polyclonal
HXB2 Location	Tat	Tat Epitope Map
Author Location	Tat( IIIB)
Epitope
Subtype	B
Ab Type
Neutralizing
Species (Isotype)	mouse(IgG)
Patient
Immunogen	vaccine
Keywords	adjuvant comparison, genital and mucosal immunity

Vaccine Details

Vaccine type	protein
Vaccine strain	B clade IIIB
Vaccine component	Tat
Adjuvant	Cholera toxin (CT), E. coli mutant heat labile enterotoxin (LT-R72), E. coli heat labile enterotoxin

Notes

Showing 1 of 1 note.

Intranasal immunization of BALB/c mice with Tat and e.coli heat-labile enterotoxin (LT) and non-toxic LT-R72 LT induced strong antigen-specific IgG Abs which remained stable for one year. Tat-specific IgA responses were measured in vaginal and intestinal secretions. Immunization of BALB/c mice with native Tat (aa1-86) induced serum IgG directed against an immunodominant epitope (aa1-20) and against a second epitope (aa 46-60). CTL responses were also observed. Anti-Tat serum Abs neutralized Tat activity in a dose-independent manner. C57BL/6 remained unresponsive to Tat immunizations when Tat was co-administered with LT or cholera toxin (CT) as adjuvant; BALB/c mice are H-2d, C57BL/6 are H-2b. Congenic BALB.C mice that express H-2b rather than H-2d also could not respond to Tat, suggesting the response to Tat is constrained by the haplotype. Marinaro2003 (adjuvant comparison, genital and mucosal immunity)

References

Showing 1 of 1 reference.

Marinaro2003 Mariarosaria Marinaro, Antonella Riccomi, Rino Rappuoli, Mariagrazia Pizza, Valeria Fiorelli, Antonella Tripiciano, Aurelio Cafaro, Barbara Ensoli, and Maria Teresa De Magistris. Mucosal Delivery of the Human Immunodeficiency Virus-1 Tat Protein in Mice Elicits Systemic Neutralizing Antibodies, Cytotoxic T Lymphocytes and Mucosal IgA. Vaccine, 21(25-26):3972-3981, 8 Sep 2003. PubMed ID: 12922133. Show all entries for this paper.