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Displaying record number 1052

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MAb ID	67G6/C4
HXB2 Location	Env	Env Epitope Map
Author Location	gp120(gp120 SF162)
Research Contact	Dr. Abraham Pinter, Public Health Research Institute, Newark, NJ, pinter@phri.org
Epitope
Subtype	B
Ab Type	gp120 CD4BS
Neutralizing
Species (Isotype)	transgenic mouse(IgG2κ)
Patient
Immunogen	vaccine
Keywords	antibody interactions, binding affinity, review, variant cross-reactivity

Vaccine Details

Vaccine type	protein
Vaccine strain	B clade SF162
Vaccine component	gp120
Adjuvant	Ribi adjuvant (MPL+TDM) (RIBI)

Notes

Showing 3 of 3 notes.

67G6/C4: This Ab did not bind to gp120IIIb, it did not prevent uptake of gp120 by APCs, and had no inhibitory effect on gp120 antigen presentation by MHC class II. Lysosomal enzyme digestion of gp120 treated with 67G6/C4 yielded digestion rate and fragmentation similar to that of gp120 alone. It is thus concluded that CD4bs Ab 67G6/C4, with low affinity to gp120, does not inhibit gp120 processing and presentation. Tuen2005 (antibody interactions, binding affinity)
67G6/C4: This review summarizes MAbs directed to HIV-1 Env. There are 51 CD4BS MAbs and Fabs in the database. Most neutralize TCLA strains only, this MAb is one of four that are non-neutralizing. Gorny2003 (review)
67G6/C4: Transgenic mice (strain XenoMouse G2) carrying human genes allowing production of fully human IgG2κ MAbs were used to rapidly create a panel of anti-HIV gp120 MAb-producing hybridomas by immunization with HIV SF162 gp120---6 anti-CD4BS MAbs competed with anti-CD4BS MAb 5145A, blocked sCD4 binding and were conformationally sensitive---4/6 could neutralize the autologous strain SF162, and were broadly cross-reactive with B clade R5 and X4 strains (not E clade)---67G6/C4 could not neutralize autologous SF162, and its binding was strain-specific. He2002 (variant cross-reactivity)

References

Showing 3 of 3 references.

He2002 Yuxian He, William J. Honnen, Chavdar P. Krachmarov, Michael Burkhart, Samuel C. Kayman, Jose Corvalan, and Abraham Pinter. Efficient Isolation of Novel Human Monoclonal Antibodies with Neutralizing Activity Against HIV-1 from Transgenic Mice Expressing Human Ig Loci. J. Immunol., 169(1):595-605, 1 Jul 2002. PubMed ID: 12077293. Show all entries for this paper.

Gorny2003 Miroslaw K. Gorny and Susan Zolla-Pazner. Human Monoclonal Antibodies that Neutralize HIV-1. In Bette T. M. Korber and et. al., editors, HIV Immunology and HIV/SIV Vaccine Databases 2003. pages 37--51. Los Alamos National Laboratory, Theoretical Biology \& Biophysics, Los Alamos, N.M., 2004. URL: http://www.hiv.lanl.gov/content/immunology/pdf/2003/zolla-pazner_article.pdf. LA-UR 04-8162. Show all entries for this paper.

Tuen2005 Michael Tuen, Maria Luisa Visciano, Peter C. Chien, Jr., Sandra Cohen, Pei-de Chen, James Robinson, Yuxian He, Abraham Pinter, Miroslaw K Gorny, and Catarina E Hioe. Characterization of Antibodies that Inhibit HIV gp120 Antigen Processing and Presentation. Eur. J. Immunol., 35(9):2541-2551, Sep 2005. PubMed ID: 16106369. Show all entries for this paper.