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Displaying record number 1009

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MAb ID	EH1
HXB2 Location	Nef(194-206) DNA(9376..9414)	Nef Epitope Map
Author Location	Nef( SF2)
Research Contact	Frank Jirik, Centre for Molecular Med and Therapeutics, U. B. C., Vancouver, B. C. Canada
Epitope	`MARELHPEYYKDC`	Epitope Alignment
Ab Type	C-term
Neutralizing
Species (Isotype)	mouse(IgG1κ)
Patient
Immunogen	vaccine
Keywords

Vaccine Details

Vaccine type	protein
Vaccine component	Nef

Notes

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EH1: The light and heavy chains of three MAbs (AG11, AE6, EH1) specific to C-terminus of NEF were cloned and variable regions sequenced -- the complementarity determining regions (CDR) of AG11 and AE6 were highly related (95.1% at the DNA level) and bound LAI Nef, but not SF2 Nef -- EH1 bound to SF2 and LAI and cross-competed AG11 and AE6 but had a distinctive CDR (57.9% similar to AG11) -- single chain Abs were constructed from AG11 and EH1 and subcloned into a eukaryotic expression vector with a green fluorescent protein marker to allow intracellular expression -- the single chain Abs bind Nef intracellularly and may be useful to better understand the role of Nef and as a gene therapy model. Chang1998

References

Showing 1 of 1 reference.

Chang1998 A. H. Chang, J. A. Hoxie, S. Cassol, M. O'Shaughnessy, and F. Jirik. Construction of Single-Chain Antibodies That Bind an Overlapping Epitope of HIV-1 Nef. FEBS Lett., 441:307-312, 1998. PubMed ID: 9883905. Show all entries for this paper.