Purpose: Make a consensus of IUPAC ambiguity codes for an alignment, or for blocks of sequences in an alignment. This tool works only for nucleotide alignments.
Usage: Browse or paste your alignment, choose options, and click "Run". You can specify the character presence percentage, which is the minimum representation required for a character to be considered in computing the consensus for that column. For example, a column containing 99 "A" and 1 "G" will be scored as "R" if the percentage is 0, but will be scored as "A" if the character presence is set at 1% or greater. In the latter case, the G would be ignored since it does not exceed 1% of the total. Note that all characters other than ACGTU are ignored. For details, see Ambiguity Consensus Maker Explanation.
