Standardized Assessments of Neutralizing Antibodies for HIV/AIDS Vaccine Development

David C. Montefiori, Duke University Medical Center


This site provides detailed information for making standardized assessments of neutralizing antibodies against HIV, SIV and SHIV using molecularly cloned Env-pseudotyped viruses in an optimized and validated luciferase (Luc) reporter gene assay. Access is provided to assay protocols and macros currently in use at the Duke Central Reference Laboratory. This site also contains educational slides, tutorials and links to relevant publications and assay reagents. Special attention is given to HIV-1 reference strains that are available as molecularly cloned Env-pseudotyped viruses.

PowerPoint Presentations:

Assay Protocol and Support Documents: Key Publications:
  • Mascola et al. 2005. Recommendations for the Design and Use of Standard Virus Panels To Assess Neutralizing Antibody Responses Elicited by Candidate Human Immunodeficiency Virus Type 1 Vaccines. J Virol. 2005 Aug;79(16):10103-7. PMID 16051803

  • Li et al. 2005. Human Immunodeficiency Virus Type 1 env Clones from Acute and Early Subtype B Infections for Standardized Assessments of Vaccine-Elicited Neutralizing Antibodies. J Virol. Aug;79(16):10108-25. PMID 16051804

  • Li et al. 2006. Genetic and Neutralization Properties of Subtype C Human Immunodeficiency Virus Type 1 Molecular env Clones from Acute and early Heterosexually Acquired Infections in Southern Africa. PMID 16971434. (Link to PDF.)

  • Seaman et al. 2010. Tiered Categorization of a Diverse Panel of HIV-1 Env Pseudoviruses for Assessment of Neutralizing Antibodies. PMID 19939925. (Link to PDF.)

Reference Reagents: Sequences:       Notes on the sequence alignment

Please send questions, comments and suggestions to Kelli Greene at:

We thank the Bill & Melinda Gates Foundation and their support of the
Collaboration for AIDS Vaccine Discovery (CAVD) - a component of the Global HIV Vaccine Enterprise.

Bill & Melinda Gates Foundation The Collaboration for AIDS Vaccine Discovery (CAVD) The Global HIV Vaccine Enterprise

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