This site provides detailed information for making standardized assessments of neutralizing antibodies against HIV, SIV and SHIV using molecularly
cloned Env-pseudotyped viruses in an optimized and validated luciferase (Luc) reporter gene assay. Access is provided to assay protocols and
macros currently in use at the Duke Central Reference Laboratory. This site also contains educational slides, tutorials and links to relevant
publications and assay reagents. Special attention is given to HIV-1 reference strains that are available as molecularly cloned Env-pseudotyped viruses.
Assay Protocol and Support Documents:
Notes on the sequence alignment
- Mascola et al. 2005. Recommendations for the Design and Use of
Standard Virus Panels To Assess Neutralizing Antibody Responses Elicited by Candidate Human Immunodeficiency Virus Type 1 Vaccines. J Virol. 2005 Aug;79(16):10103-7. PMID 16051803
Li et al. 2005. Human Immunodeficiency Virus Type 1 env Clones from Acute and Early Subtype B Infections for Standardized Assessments of Vaccine-Elicited Neutralizing Antibodies. J Virol. Aug;79(16):10108-25.
- Li et al. 2006. Genetic and Neutralization Properties of Subtype C Human Immunodeficiency Virus Type 1 Molecular env Clones from Acute and early Heterosexually Acquired Infections in Southern Africa.
(Link to PDF.)
- Seaman et al. 2010. Tiered Categorization of a Diverse Panel of HIV-1 Env Pseudoviruses for Assessment of Neutralizing Antibodies.
(Link to PDF.)