HIV molecular immunology database

 

Summary of Best Neutralizing Antibodies

Download summary of best neutralizing antibodies as CSV or XLS files.

This is a list of most broadly neutralizing HIV antibodies, with links to papers, Ab sequences and structure, notes on breadth of neutralization, Ab contact or key residues and heavy and light chain composition. Note: this is a work in progress, so not all relevant papers and antibodies are listed.

See also: Neutralizing Antibody Resources


  Mab Binding site Author
Journal
Pmid
First paper Breadth of neutralization with IC50<50 μg/ml Breadth of neutralization with IC80 or IC90<50 μg/ml Structure, PDB ID Ab sequence Heavy chain Light chain Germline Ab sequence Ab binding affinity Listings of antibody contact or key residues

VRC01 CD4bs Wu2010
Science
20616233
YES 91% of 190 isolates, representing major HIV-1 clades 86% of 190 isolates, representing major HIV-1 clades, with IC80   GI:294875838 -- heavy chain variable region

GI:294875848 -- light chain variable region
V: IGHV1-02*02
D: IGHD3-16*01 (or *02)
J: IGHJ1*01 or IGHJ2*01
V: IGKV3-11*01
J: IGKJ2*01
Fig. S5 Bound strongly to RSC3 and gp120 and weakly to ΔRSC3, Fig. 2 and S4.  
   Zhou2010
Science
20616231
      3NGB  

 
    Fig. S12 Figs. 5, 6, S3 Env, defined by crystal structure: Fig S1. Antibody, defined by crystal structure: Fig. S9
   Wu2011
Science
21835983
         

 
    Sequence, Figs. 1, S14, S18. Phylogenic analysis, Fig. 5, Fig. S13   Antibody, defined by crystal structure, compared to key residues of other CD4bs antibodies, Fig. S4.
   Scheid2011
Science
21764753
  100% of 118 isolates representing major HIV-1 clades      

 
      Fig. 3, Table S9. Antibody, defined by crystal structure in Zhou2010, Fig. 4, Fig. S3, and Fig. S4 provide comparisons with other CD4bs Nabs.
   Walker2011
Nature
21849977
  93% of 162 isolates representing major HIV clades 89% of 162 isolates representing major HIV clades, with IC90    

 
         
   Diskin2011
Science
22033520
         

 
        Antibody, sequence and gp120-bound structures compared to NIH45-46, Figs. S1, S2.
   Falkowska2011
J Virol
22345481
         

 
        Env, Alanine scanning neutralization impact, Table 3
   McLellan2011
Nature
22113616
  89% of 178 isolates representing major HIV-1 clades      

 
        Env, Alanine scanning neutralization impact, Table 3
   Huang2012a
Nature
  89% of 180 isolates representing major HIV-1 clades at IC50<50 μg/ml, and 75% at IC50 < 1 μg/ml.      

 
         

VRC02 CD4bs Wu2010
Science
20616233
YES 91% of 190 isolates, representing major HIV-1 clades 88% of 190 isolates, representing major HIV-1 clades, with IC80   GI:294875840 -- heavy chain variable region

GI:294875850 -- light chain variable region
V: IGHV1-02*02
D: IGHD3-16*01 (or *02)
J: IGHJ1*01 or IGHJ2*01
V: IGKV3-11*01
J: IGKJ2*01
Fig. S5 Bound strongly to RSC3 and gp120 and weakly to ΔRSC3, Fig. 2 and S4.  

VRC03 CD4bs Wu2010
Science
20616233
YES 57% of 190 isolates, representing major HIV-1 clades 48% of 190 isolates, representing major HIV-1 clades, with IC80   GI:294875842 -- heavy chain variable region

GI:294875853 -- light chain variable region
V: IGHV1-02*02
D: IGHD3 family
J: IGHJ1*01
V: IGKV3-20*01
J: IGKJ2*01
Fig. S5 Bound strongly to RSC3 and weakly to ΔRSC3, affinity to gp120 was 10-fold lower than that of VRC01 and VRC02, Fig. 2 and S4.  
   Wu2011
Science
21835983
      3SE8  

 
        Antibody, defined by crystal structure, compared to key residues of other CD4bs antibodies, Fig. S4.

VRC-PG04 or PGV04 CD4bs Wu2011
Science
21835983
YES 76% of 178 isolates, representing major HIV-1 clades 74% of 178 isolates, representing major HIV-1 clades, with IC80 3SE9 GI:343794446 -- heavy chain variable region

GI:343794450 -- light chain variable region
V: IGHV1-02*02
D: IGHD5-12*01
J: IGHJ2*01
V: IGKV3-20*01
J: IGKJ5*01
Phylogenic analysis, Figs. 5, 6, Fig. S12   Antibody, defined by crystal structure, compared to key residues of other CD4bs antibodies, Fig. S4.
   Falkowska2011
J Virol
22345481
  88% of 162 isolates representing major HIV clades      

 
        Env: defined by crystal structure and Alanine scanning, Table 3 (neutralization impact) and Table 4 (binding affinity), Structure mapping of alanine substitutions, Fig. 5.
   Walker2011
Nature
21849977
  88% of 162 isolates representing major HIV clades 77% of 162 isolates representing major HIV clades, with IC90    

 
         

VRC-PG04b CD4bs Wu2011
Science
21835983
YES 71% of 178 isolates, representing major HIV-1 clades 64% of 178 isolates, representing major HIV-1 clades, with IC80   GI:343794448 -- heavy chain variable region

GI:343794452 -- light chain variable region
V: IGHV1-02*02
D: IGHD5-12*01
J: IGHJ2*01
V: IGKV3-20*01
J: IGKJ5*01
     

VRC-CH31 CD4bs Wu2011
Science
21835983
YES 83% of 80 isolates, representing major HIV-1 clades, and 85% of 20 clade A, B and C Env-pseudoviruses 80% of 80 isolates, representing major HIV-1 clades, and 85% of 20 clade A, B and C Env-pseudoviruses, with IC80   GI:343794388 -- heavy chain variable region

GI:343794394 -- light chain variable region
V: IGHV1-02*02
D: IGHD3-16*01
J: IGHJ4*02
V: IGKV1-33*01
J: IGKJ2*01
     
   Wu2011
J Virol
22301150
  84% of 96 isolates from major clades      

 
         

VRC-CH30 CD4bs Wu2011
Science
21835983
YES 85% of 20 clade A, B and C Env-pseudoviruses 80% of 20 clade A, B and C Env-pseudoviruses, with IC80   GI:343794386 -- heavy chain variable region

GI:343794392 -- light chain variable region
V: IGHV1-02*02
D: IGHD3-16*01
J: IGHJ4*02
V: IGKV1-33*01
J: IGKJ2*01
     

VRC-CH32 CD4bs Wu2011
Science
21835983
YES 80% of 20 clade A, B and C Env-pseudoviruses 75% of 20 clade A, B and C Env-pseudoviruses, with IC80   GI:343794390 -- heavy chain variable region

GI:343794396 -- light chain variable region
V: IGHV1-02*02
D: IGHD3-16*01
J: IGHJ4*02
V: IGKV1-33*01
J: IGKJ2*01
     

VRC-CH33 CD4bs Wu2011
Science
21835983
YES 90% of 20 clade A, B and C Env-pseudoviruses 80% of 20 clade A, B and C Env-pseudoviruses, with IC80   GI:343794462 -- heavy chain variable region

GI:343794458 -- light chain variable region
V: IGHV1-02*02
D: IGHD3-16*01
J: IGHJ4*02
V: IGKV1-33*01
J: IGKJ2*01
     

VRC-CH34 CD4bs Wu2011
Science
21835983
YES 80% of 20 clade A, B and C Env-pseudoviruses 75% of 20 clade A, B and C Env-pseudoviruses, with IC80   GI:343794464 -- heavy chain variable region

GI:343794460 -- light chain variable region
V: IGHV1-02*02
D: IGHD3-16*01
J: IGHJ4*02
V: IGKV1-33*01
J: IGKJ2*01
     

IgG1b12 or b12 CD4bs Wu2010
Science
20616233
  41% of 190 isolates, representing major HIV-1 clades 25% of 190 isolates, representing major HIV-1 clades, with IC80    

 
V: IGHV1-03*01
D: IGHD3-10*02
J: IGHJ6*03
V: IGKV3-20*01
J: IGKJ2*01
  Figs. 2 and S4.  
   Barbas1993
J Mol Biol
8478936
        sequences in the paper

 
V: IGHV1-3*01
D: IGHD2-21
J: IGHJ6
       
   Burton1991
PNAS
1719545
YES        

 
         
   Zhou2007
Nature
17301785
      2NY7  

 
        Antibody, Alanine scanning affinity impact, Table S6. Crystal structure contacts, Fig. S3. Env, Alanine scanning affinity impact, Table S7. Crystal structure contacts, Fig. S4.
   Binley2004
J Virol
15542675
  50% of 90 isolates representing major HIV-1 clades 34% of 90 isolates representing major HIV-1 clades, with IC90    

 
         
   Walker2009
Science
19729618
  35% of 162 isolates representing major HIV-1 clades 19% of 162 isolates representing major HIV-1 clades, with IC90    

 
         
   Corti2010
PLOS ONE
20098712
  47% of 92 isolates representing major HIV-1 clades      

 
      Fig. 3  
   Falkowska2011
J Virol
22345481
         

 
        Env, Alanine scanning neutralization impact, Table 3
   Walker2011
Nature
21849977
  34% of 162 isolates representing major HIV clades 19% of 162 isolates representing major HIV clades, with IC90    

 
      Fig. S4  
   Burton1994
Science
7973652
  7/10 US isolates completely neutralized and all 10 were 50% neutralized; 10/14 additional isolates 50% neutralized.      

 
         
   Bonsignori2011
J Virol
21795340
         

 
      Table 2, Fig. S5  

2F5 gp41 adjacent to cluster II, C-term, gp41 MPER Buchacher1994
AIDSR Res Hum Retroviruses
7520721
YES        

 
         
   Bryson2009
J Virol
19740978
      22 structures  

 
         
   Julien2008
J Mol Biol
18824005
      8 structures  

 
         
   delaArada2009
J Phys Chem
19754136
      2 structures  

 
         
   Ofek2010
PNAS
20876137
      3LEV  

 
         
   Kunert1998
AIDS Res Hum Retroviruses
9737583
        sequences in the paper

 
V: IGHV2
J: IGHJ6
V: IGKV1
J: IGKJ4
Tables 2, 3, 4.    
   Corti2010
PLOS ONE
20098712
  39% of 92 isolates representing major HIV-1 clades      

 
         
   Walker2009
Science
19729618
  60% of 162 isolates representing major HIV-1 clades 33% of 162 isolates representing major HIV-1 clades, with IC90    

 
         
   Binley2004
J Virol
15542675
  67% of 90 isolates representing major HIV-1 clades 43% of 90 isolates representing major HIV-1 clades, with IC90    

 
         
   McLellan2011
Nature
22113616
  57% of 178 isolates representing major HIV-1 clades      

 
         
   Huang2012a
Nature
  57% of 180 isolates representing major HIV-1 clades at IC50<50 μg/ml, and 16% at IC50 < 1 μg/ml.      

 
         

2G12 gp120 carbohydrates at glycosylation residues in C2, C3, C4, and V4 Buchacher1994
AIDSR Res Hum Retroviruses
7520721
YES        

 
         
   Menendez2008
FASEB J
18198210
      3MUH  

 
         
   Doores2010
J Virol
20702629
      3OAU  

 
         
   Calarese2003
Science
12829775
      3 structures  

 
         
   Calarese2005
PNAS
16174734
      4 structures  

 
         
   Corti2010
PLOS ONE
20098712
  28% of 92 isolates representing major HIV-1 clades      

 
         
   Kunert1998
AIDS Res Hum Retroviruses
9737583
        sequences in the paper

 
V: IGHV3
J: IGHJ3
V: IGKV1
J: IGKJ2
Tables 2, 3, 4.    
   Walker2009
Science
19729618
  32% of 162 isolates representing major HIV-1 clades 15% of 162 isolates representing major HIV-1 clades, with IC90    

 
         
   Wu2011
Science
21835983
         

 
V: IGHV3-21*01/2/3/4 V: IGKV1-5*03      
   Falkowska2011
J Virol
22345481
         

 
        Env, Alanine scanning binding affinity impact, Table 4
   Binley2004
J Virol
15542675
  41% of 90 isolates representing major HIV-1 clades 21% of 90 isolates representing major HIV-1 clades, with IC90    

 
         
   Walker2011
Nature
21849977
  32% of 162 isolates representing major HIV clades 15% of 162 isolates representing major HIV clades, with IC90    

 
      Figs. S4, S5, S6  
   McLellan2011
Nature
22113616
  27% of 178 isolates representing major HIV-1 clades      

 
         

4E10 C-term, gp41 MPER Buchacher1994
AIDSR Res Hum Retroviruses
7520721
YES        

 
         
   Cardoso2005
Immunity
15723805
      4 structures  

 
         
   Cardoso2007

17125793
      3 structures  

 
         
   Correia2009
Structure
20826338
      6 structures  

 
         
   Kunert2004
AIDS Res Hum Retroviruses
15307922
        sequences in the paper, Fig. 1

 
V: IGVH1-69
D: IGHD3-16
J: IGHJ1
  Fig. 1, Table 1    
   Corti2010
PLOS ONE
20098712
  98% of 92 isolates representing major HIV-1 clades      

 
         
   Walker2009
Science
19729618
  98% of 162 isolates representing major HIV-1 clades 36% of 162 isolates representing major HIV-1 clades, with IC90    

 
         
   Binley2004
J Virol
15542675
  100% of 90 isolates representing major HIV-1 clades 56% of 90 isolates representing major HIV-1 clades, with IC90    

 
         
   Walker2011
Nature
21849977
  96% of 162 isolates representing major HIV clades 34% of 162 isolates representing major HIV clades, with IC90    

 
         
   Huang2012a
Nature
  98% of 180 isolates representing major HIV-1 clades at IC50<50 μg/ml, and 37% at IC50 < 1 μg/ml.      

 
         

10E8 C-term, gp41 MPER Huang2012a
Nature
YES 98% of 180 isolates representing major HIV-1 clades at IC50<50 μg/ml, and 72% at IC50 < 1 μg/ml.   4G6F GI:JX645769.1 -- heavy chain variable region

GI:JX645770.1 -- light chain variable region
V: IGHV3-15*05
D: IGHD3-3*01
J: IGHJ1*01
V: IGLV3-19*01
J: IGLJ3*02
Figure S1. Figures S3, S4, S6, Table S15 Env alanine scanning, Figures 2, S8, Table S2, S3. Env contact residues, Fab 10E8 structure with its MPER epitope, Figure 4.

PG9 V2-V3 loops, quaternary structure Walker2009
Science
19729618
YES 79% of 162 isolates representing major HIV-1 clades 51% of 162 isolates representing major HIV-1 clades, with IC90   GI:281185524 -- heavy chain variable region

GI:281185534 -- light chain variable region
V: IGVH3-33*05 V: IGVL2-14*01 Table S2. Figs. 1, S4, S7, S8, S10.  
   Pejchal2010
PNAS
20534513
      3MUH  

 
      Fig. 3. Antibody contact residues, predicted from homology modeling, Fig. S2.
   McLellan2011
Nature
22113616
  78% of 178 isolates representing major HIV-1 clades   3U4E, 3U36, 3U2S  

 
      Figs. S4, S15 Ab contact residues, Table S12 and S13. End alanine scanning neutralization impact, Table S14. Env contact residues, V1V2 scaffold crystal structure, Fig. 2, Table S9, Table S10
   Walker2011
Nature
21849977
  77% of 162 isolates representing major HIV clades 46% of 162 isolates representing major HIV clades, with IC90    

 
      Figs. S4, S5  
   Bonsignori2011
J Virol
21795340
         

 
      Figs. 3, S5  
   Davenport2011
J Virol
21543501
         

 
      Figs. 1, 3, 5, Table 1.  
   Huang2012a
Nature
  78% of 180 isolates representing major HIV-1 clades at IC50<50 μg/ml, and 65% at IC50 < 1 μg/ml.      

 
         

PG16 V2-V3 loops, quaternary structure Walker2009
Science
19729618
YES 73% of 162 isolates representing major HIV-1 clades 35% of 162 isolates representing major HIV-1 clades, with IC90   GI:281185522 -- heavy chain variable region

GI:281185532 -- light chain variable region
V: IGVH3-33*05 V: IGVL2-14*01 Table S2. Figs. 1, S4, S7, S8, S10.  
   McLellan2011
Nature
22113616
  74% of 178 isolates representing major HIV-1 clades      

 
      Fig. S15 Ab contact residues, Table S12 and S13. Model based on PG9. PG16 paratope by arginine-scanning mutagenesis, Fig. S14.
   Pejchal2010
PNAS
20534513
      3MUG  

 
      Fig. 3 Antibody, impact of amino acid substitutions on neutralization and binding, Table 2.
   Pancera2010
J Virol
20538861
      3MME, 3LRS  

 
         
   Bonsignori2011
J Virol
21795340
         

 
      Figs. 3  
   Davenport2011
J Virol
21543501
         

 
      Figs. 1, 3, 5.  
   Huang2012a
Nature
  73% of 180 isolates representing major HIV-1 clades at IC50<50 μg/ml, and 59% at IC50 < 1 μg/ml.      

 
         

HJ16 CD4bs Corti2010
PLOS ONE
20098712
YES 36% of 92 isolates representing major HIV-1 clades      

 
      Fig. 3  
   Wu2011
Science
21835983
         

 
V: IGHV3-30*18 V: IGKV4-1*01      

3BNC117 CD4bs Scheid2011
Science
21764753
YES 100% of 118 isolates representing major HIV-1 clades, and 1/5 VRC01-resistant isolates. 95% of 118 isolates representing major HIV-1 clades   GI:346983261 -- heavy chain variable region

GI:346983263 -- light chain variable region
V: IGVH1-2
D: IGHD6-25/2-8
J: IGHJ2/6
V: IGKV1D-33
J: IGKJ3
Sequences, Figs. 4, S13; Phylogenetic analysis Fig. S12. High affinity binder, Figs. 3, S10, Table S9  
   Huang2012a
Nature
  84% of 180 isolates representing major HIV-1 clades at IC50<50 μg/ml, and 77% at IC50 < 1 μg/ml.      

 
         

3BNC55 Scheid2011
Science
21764753
YES 81% of 118 isolates representing major HIV-1 clades, and 2/5 VRC01-resistant isolates. 76% of 118 isolates representing major HIV-1 clades    

 
V: IGVH1-2
D: IGHD3-3/6-19/5-12
J: IGHJ2/6
V: IGKV1D-33
J: IGKJ1/3
Phylogenetic analysis Fig. S12. Lower affinity to YU2-gp140 trimer than 3BNC117, Fig. S10, Table S9.  

NIH45-46 CD4bs Scheid2011
Science
21764753
YES 100% of 118 isolates representing major HIV-1 clades 100% of 118 isolates representing major HIV-1 clades   GI:346983273 -- heavy chain variable region

GI:346983275 -- light chain variable region
V: IGVH1-2 V: IgVK3-11 Sequences, Figs. 4, S13; Phylogenetic analysis Fig. S12. Fig. 3, Table S9.  
   Diskin2011
Science
22033520
  NIH45-46_G54W was significantly more potent than NIH45-46, based on a panel of 82 isolates from all clades, both with IC50 and IC80   3U7Y, 3U7W  

 
      Fig. S7 Antibody, crystal structure contact and key residues, Figs. 1, 2. Sequence and gp120-bound structures compared to VRC01, Figs. S1, S2. Impact of amino acid substitutions on neutralization, Table S2. Env, impact of amino acid substitutions on neutralization, Table S7.
   Huang2012a
Nature
  85% of 180 isolates representing major HIV-1 clades at IC50<50 μg/ml, and 76% at IC50 < 1 μg/ml.      

 
         

12A12 CD4bs Scheid2011
Science
21764753
YES 100% of 118 isolates representing major HIV-1 clades, and 1/5 VRC01-resistant isolates. 95% of 118 isolates representing major HIV-1 clades   GI:346983273 -- heavy chain variable region

GI:346983275 -- light chain variable region
V: IGVH1-2
D: IGHD1-26/3-10
J: IGHJ4/5
V: IGKV1D-33
J: IGKJ3
Sequences, Figs. 4, S13; Phylogenetic analysis Fig. S12. Fig. 3, Table S9.  

8ANC195 Scheid2011
Science
21764753
YES 57% of 118 isolates representing major HIV-1 clades, and 2/5 VRC01-resistant isolates. 57% of 118 isolates representing major HIV-1 clades   GI:346983293 -- heavy chain variable region

GI:346983295 -- light chain variable region
V: IGVH1-69
D: IGHD3-3
V: IGKV1D-33
J: IGKJ3
Sequences, Fig. 4; Phylogenetic analysis Fig. S12. Table S9  

3BNC60 CD4bs Scheid2011
Science
21764753
YES 15/15 isolates and 1/5 VRC01-resistant isolates.   3RPI GI:346983257 -- heavy chain variable region

GI:346983259 -- light chain variable region
V: IGVH1-2
D: IGHD6-25/3-3
J: IGHJ2/6
V: IGKV1-5
J: IG1/5
Sequences, Figs. 4, S13; Phylogenetic analysis Fig. S12. High affinity binder, Figs. 3, S10, Table S9  

3BNC62 Scheid2011
Science
21764753
YES 11/15 isolates at concentration < 15 μg/ml      

 
V: IGVH1-2
D: IGHD6-25/6-13/6-6
J: IGHJ2/6
V: IGKV1D-33
J: IGKJ3
Phylogenetic analysis Fig. S12.    

12A21 CD4bs Scheid2011
Science
21764753
YES 13/15 isolates and 1/5 VRC01-resistant isolates.     GI:346983269 -- heavy chain variable region

GI:346983271 -- light chain variable region
V: IGVH1-2
D: IGHD5-12/3-10
J: IGHJ4/5
V: IGKV1D-33
J: IGKJ3
Sequences, Figs. 4, S13; Phylogenetic analysis Fig. S12. Fig. 3, Table S9.  

PGT121 V3 loop Walker2011
Nature
21849977
YES 70% of 162 isolates representing major HIV clades 55% of 162 isolates representing major HIV clades, with IC90   GI:344323216 -- heavy chain variable region

GI:344323250 -- light chain variable region
V: IGHV4-59*01
J: IGHJ6*03
V: IGLV3-21*02
J: IGLJ3*02
  Bound to monomeric gp120, Table S5, Fig. S6 Env, Alanine scanning neutralization impact, Table S6.
   Mouquet2012a
PNAS
23115339
  63% of 119 difficult to neutralize pseudoviruses representing major HIV clades 57% of 119 difficult to neutralize pseudoviruses representing major HIV clades 4FQQ, 4FQC, 4FQ1  

 
    Sequences, phylogeny Fig. S1 Figs. S2-S8. Contacts between the glycan and the PGT121 VH domain, Fig S17; contacts between PFT121 Fab and bound glycan, Table S9. Neutralization sensitivity according to N332 PNGS, Table S5.

10-1074 V3 loop Mouquet2012a
PNAS
23115339
YES 57% of 119 difficult to neutralize pseudoviruses representing major HIV clades 56% of 119 difficult to neutralize pseudoviruses representing major HIV clades 4FQ2  

 
V: IGVH4-59
D: IGHD3-3/16
J: IGHJ6
V: IGLV3-21
J: IGLJ3
Sequences, phylogeny Fig. S1 Figs. S2-S8. Neutralization sensitivity according to N332 PNGS, Table S5.

PGT122 V3 loop Walker2011
Nature
21849977
YES 65% of 162 isolates representing major HIV clades 54% of 162 isolates representing major HIV clades, with IC90   GI:344323218 -- heavy chain variable region

GI:344323252 -- light chain variable region
V: IGHV4-59*01
J: IGHJ6*03
V: IGLV3-21*02
J: IGLJ3*02
  Bound to monomeric gp120, Table S5, Fig. S6 Env, Alanine scanning neutralization impact, Table S6.

PGT123 V3 loop Walker2011
Nature
21849977
YES 67% of 162 isolates representing major HIV clades 52% of 162 isolates representing major HIV clades, with IC90   GI:344323220 -- heavy chain variable region

GI:344323254 -- light chain variable region
V: IGHV4-59*01
J: IGHJ6*03
V: IGLV3-21*02
J: IGLJ3*02
  Bound to monomeric gp120, Table S5, Fig. S6  

PGT125 V3 loop Walker2011
Nature
21849977
YES 52% of 162 isolates representing major HIV clades 37% of 162 isolates representing major HIV clades, with IC90    

GI:344323256 -- light chain variable region
V: IGHV4-39*07
J: IGHJ5*02
V: IGLV2-8*01
J: IGLJ2*01 or IGLJ3*01
  Bound to monomeric gp120, Table S5, Fig. S6 Env, Alanine scanning neutralization impact, Table S6.

PGT126 V3 loop Walker2011
Nature
21849977
YES 60% of 162 isolates representing major HIV clades 45% of 162 isolates representing major HIV clades, with IC90    

GI:344323258 -- light chain variable region
V: IGHV4-39*07
J: IGHJ5*02
V: IGLV2-8*01
J: IGLJ2*01 or IGLJ3*01
  Bound to monomeric gp120, Table S5, Fig. S6 Env, Alanine scanning neutralization impact, Table S6.

PGT127 V3 loop Walker2011
Nature
21849977
YES 50% of 162 isolates representing major HIV clades 36% of 162 isolates representing major HIV clades, with IC90   GI:344323226 -- heavy chain variable region

GI:344323260 -- light chain variable region
V: IGHV4-39*07
J: IGHJ5*02
V: IGLV2-8*01
J: IGLJ2*01 or IGLJ3*01
  Bound to monomeric gp120, Table S5, Fig. S6 Env, Alanine scanning neutralization impact, Table S6.
   Pejchal2011
Science
21998254
      3TWC  

 
      Figs. 3, 5, S14 Antibody, crystal structure: Fig. S1, Table S2. Env, amino acid substitutions neutralization impact, Fig. S10.

PGT128 V3 loop Walker2011
Nature
21849977
YES 72% of 162 isolates representing major HIV clades 57% of 162 isolates representing major HIV clades, with IC90   GI:344323228 -- heavy chain variable region

GI:344323262 -- light chain variable region
V: IGHV4-39*07
J: IGHJ5*02
V: IGLV2-8*01
J: IGLJ2*01 or IGLJ3*01
  Bound to monomeric gp120, Table S5, Fig. S6 Env, Alanine scanning neutralization impact, Table S6.
   Pejchal2011
Science
21998254
      3TYG, 3TV3  

 
      Figs. 3, 5, S14 Antibody, crystal structure, Fig. 1, 2, Tables S2-S4. Env, amino acid substitutions neutralization impact, Fig. S10.

PGT130 V3 loop Walker2011
Nature
21849977
YES 52% of 162 isolates representing major HIV clades 28% of 162 isolates representing major HIV clades, with IC90   GI:344323230 -- heavy chain variable region

GI:344323264 -- light chain variable region
V: IGHV4-39*07
J: IGHJ5*02
V: IGLV2-8*01
J: IGLJ2*01 or IGLJ3*01
  Bound to monomeric gp120, Table S5, Fig. S6 Env, Alanine scanning neutralization impact, Table S6.

PGT131 V3 loop Walker2011
Nature
21849977
YES 40% of 162 isolates representing major HIV clades 19% of 162 isolates representing major HIV clades, with IC90   GI:344323232 -- heavy chain variable region

GI:344323266 -- light chain variable region
V: IGHV4-39*07
J: IGHJ5*02
V: IGLV2-8*01
J: IGLJ2*01 or IGLJ3*01
  Bound to monomeric gp120, Table S5, Fig. S6 Env, Alanine scanning neutralization impact, Table S6.

PGT135 V3 loop Walker2011
Nature
21849977
YES 33% of 162 isolates representing major HIV clades 17% of 162 isolates representing major HIV clades, with IC90   GI:344323234 -- heavy chain variable region

GI:344323268 -- light chain variable region
V: IGHV4-39*07
J: IGHJ5*02
V: IGKV3-15*01
J: IGLJ1*01
  Bound to monomeric gp120, Table S5, Fig. S6 Env, Alanine scanning neutralization impact, Table S6.

PGT141 V2-V3 loops, quaternary structure Walker2011
Nature
21849977
YES 56% of 162 isolates representing major HIV clades 35% of 162 isolates representing major HIV clades, with IC90   GI:344323240 -- heavy chain variable region

GI:344323274 -- light chain variable region
V: IGHV1-8*01
J: IGHJ6*02
V: IGKV2-28*01 or IGKV2D-28*01
J: IGKJ1*01
  Strong preference for membrane-bound, trimeric HIV - quaternary epitope, Table S5, Figs. S4, S5 Env, amino acid substitutions binding impact, Fig. S5

PGT142 V2-V3 loops, quaternary structure Walker2011
Nature
21849977
YES 57% of 162 isolates representing major HIV clades 40% of 162 isolates representing major HIV clades, with IC90   GI:344323242 -- heavy chain variable region

GI:344323276 -- light chain variable region
V: IGHV1-8*01
J: IGHJ6*02
V: IGKV2-28*01 or IGKV2D-28*01
J: IGKJ1*01
  Strong preference for membrane-bound, trimeric HIV - quaternary epitope, Table S5, Figs. S4, S5 Env, amino acid substitutions binding impact, Fig. S5

PGT143 V2-V3 loops, quaternary structure Walker2011
Nature
21849977
YES 56% of 162 isolates representing major HIV clades 33% of 162 isolates representing major HIV clades, with IC90   GI:344323244 -- heavy chain variable region

GI:344323278 -- light chain variable region
V: IGHV1-8*01
J: IGHJ6*02
V: IGKV2-28*01 or IGKV2D-28*01
J: IGKJ1*01
  Strong preference for membrane-bound, trimeric HIV - quaternary epitope, Table S5, Figs. S4, S5 Env, amino acid substitutions binding impact, Fig. S5

PGT144 V2-V3 loops, quaternary structure Walker2011
Nature
21849977
YES 38% of 162 isolates representing major HIV clades 14% of 162 isolates representing major HIV clades, with IC90   GI:344323246 -- heavy chain variable region

GI:344323280 -- light chain variable region
V: IGHV1-8*01
J: IGHJ6*02
V: IGKV2-28*01 or IGKV2D-28*01
J: IGKJ1*01
  Strong preference for membrane-bound, trimeric HIV - quaternary epitope, Table S5, Figs. S4, S5 Env, amino acid substitutions binding impact, Fig. S5

PGT145 V2-V3 loops, quaternary structure Walker2011
Nature
21849977
YES 78% of 162 isolates representing major HIV clades 53% of 162 isolates representing major HIV clades, with IC90   GI:344323248 -- heavy chain variable region

GI:344323282 -- light chain variable region
V: IGHV1-8*01
J: IGHJ6*02
V: IGKV2-28*01 or IGKV2D-28*01
J: IGKJ1*01
  Strong preference for membrane-bound, trimeric HIV - quaternary epitope, Table S5, Figs. S4, S5 Env, amino acid substitutions binding impact, Fig. S5.
   McLellan2011
Nature
22113616
      3U1S  

 
        Antibody, unbound structure, Fig. S18.

CH01 V1-V2, V2-V3, quaternary structure Bonsignori2011
J Virol
21795340
YES 46% of 91 isolates from major clades     GA: JQ267523 -- heavy chain variable region

GA:JQ267519 -- light chain variable region
V: IGHV3-20
D: IGHD3-10
J: IGHJ2
V: IGKV3-20
J: IGKJ1
Sequence, Fig. S2; Phylogenetic analysis Fig. S3. Table 2, Figs. 3, S5  
   Bonsignori2012
J Virol
22301150
  45% of 96 isolates from major clades      

 
         

CH02 V1-V2, V2-V3, quaternary structure Bonsignori2011
J Virol
21795340
YES 36% of 91 isolates from major clades     GA: JQ267524 -- heavy chain variable region

GA: JQ267520 -- light chain variable region
V: IGHV3-20
D: IGHD3-10
J: IGHJ2
V: IGKV3-20
J: IGKJ1
Sequence, Fig. S2; Phylogenetic analysis Fig. S3. Table 2, Figs. 3, S5  

CH03 V1-V2, V2-V3, quaternary structure Bonsignori2011
J Virol
21795340
YES 45% of 91 isolates from major clades     GA: JQ267525 -- heavy chain variable region

GA: JQ267521 -- light chain variable region
V: IGHV3-20
D: IGHD3-10
J: IGHJ2
V: IGKV3-20
J: IGKJ1
Sequence, Fig. S2; Phylogenetic analysis Fig. S3. Table 2, Figs. 3, S5  

CH04 V1-V2, V2-V3, quaternary structure Bonsignori2011
J Virol
21795340
YES 45% of 91 isolates from major clades     GA: JQ267526 -- heavy chain variable region

GA: JQ267522 -- light chain variable region
V: IGHV3-20
D: IGHD3-10
J: IGHJ2
V: IGKV3-20
J: IGKJ1
Sequence, Fig. S2; Phylogenetic analysis Fig. S3. Table 2, Figs. 3, S5  
   McLellan2011
Nature
22113616
      3TCL, 3U4B, 3U46  

 
        Antibody, unbound structures, Fig. S17.

CH103 CD4bs Liao2013
Nature
23552890
YES 55% of 196 isolates representing major HIV-1 clades   4JAN, 4JAM GI:482671912 -- heavy chain variable region

In the paper, Table S4.
V: IGHV4-59*01
D: IGHD3-16*01
J: IGHJ4*02
V: IGLV3-1*01
J: IGLJ1*01
Figure S7, Table S4 Table S4 Antibody paratope, crystal structure, critical residues, Figure S7, Table S12.

CH98 CD4bs Bonsignori2014
J Clin Invest
24614107
YES 64% of 42 isolates (4 tier-A1, 4 tear-1B, 34 tier-2, including 9 transmitted/founder viruses), Fig. 1     Sequence in the paper, figures S3, S4

 
V: IGHV3-30
D: IGHD1-IR1
J: IGH4
V: IGLV2-23
J: IGLJ3
Figure S3, S4 Fig. S7, S8 Point mutations, Fig. 2, structure prediction, Table S4.

PGT151 gp120-gp41 quartenary interface Bonsignori2014
J Clin Invest
24614107
YES 66% of 117 cross-clade isolates 44% of 117 cross-clade isolates    

 
V: IGHV3-30*04
J: IGHJ6*02
V: IGKV2D-29*02
J: IGKJ4*01
    Tables 2 and S6, effect of single-amino-scid substitutions on PGT151 neutralization
   Blattner2014
Immunity
24768348
      4NUG Fig. S2

 
        Substitutions affecting binding, Fig. 4.

PGT152 gp120-gp41 quartenary interface Blattner2014
Immunity
24768348
YES 64% of 117 cross-clade isolates 47% of 117 cross-clade isolates    

 
V: IGHV3-30*04
J: IGHJ6*02
V: IGKV2D-29*02
J: IGKJ4*01
     
   Blattner2014
Immunity
24768348
      4NUJ Fig. S2

 
        Substitutions affecting binding, Fig. 4.

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